JFS Abstract Details


Title Distribution of Catechins, Theaflavins, Caffeine, and Theobromine in 77 Teas Consumed in the United States
Abstract Distribution of Catechins Theaflavins Caffeine and Theobromine in 77 Teas Consumed in the United States Mendel Friedman 1 Soo-Yeun Kim 1 Sin-Jung Lee 1 Gyeong-Phil Han 1 Jae-Sook Han 1 Kap-Rang Lee 1 and Nobuyuke Kozukue 1 1 Author Friedman is with Western Regional Research Center Agricultural Research Service US Dept of Agriculture Albany CA 94710 Authors Kim S-J Lee and K-R Lee are with College of Human Ecology Yeungnam Univ Gyongsan Korea Authors Phil Han and Kozukue are with Dept of Food Service Industry Uiduck Univ Gyongbuk Korea Direct inquiries to author Friedman (mfried@pwusdagov) Keywords: HPLC catechins theaflavins caffeine theobromine teas Camelia sinensis References Beecher GR 2003 Overview of dietary flavonoids: nomenclature occurrence and intake J Nutr 133 : 3248S 54S Beecher GR Warden BA Merken H 1999 Analysis of tea polyphenols Proc Soc Exp Biol Med 220 : 267 70 Bonoli M Colabufalo P Pelillo M Gallina Toschi T Lercker G 2003 Fast determination of catechins and 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Zhao WH Shimamura T 2004 Different susceptibilities of Staphylococcus and Gram-negative rods to epigallocatechin gallate J Infect Chemother 10 : 55 8 Zhu X Chen B Ma M Luo X Zhang F Yao S Wan Z Yang D Hang H 2004 Simultaneous analysis of theanine chlorogenic acid purine alkaloids and catechins in tea samples with the help of multi-dimension information of on-line high performance liquid chromatographyelectrospray-mass spectrometry J Pharm Biomed Anal 34 : 695 704 ABSTRACT: To help define the composition of commercial teas consumed in the United States we have developed and validated an high-performance liquid chromatography (HPLC) method to analyze levels of catechins theaflavins and alkaloids in 77 commercial black green specialty (brown rice white oolong) and herbal teas extracted with hot water to simulate home use The following 13 compounds were separated in a single analysis on an Inertsil ODS-3v column with acetonitrilepotassium dihydrogen phosphate as the mobile phase: ()-epigallocatechin ()-catechin ()-epicatechin ()-epigallocatechin-3-gallate ()-gallocatechin-3-gallate ()-epicatechin-3-gallate ()-catechin-3-gallate theaflavin theaflavin-3-gallate theaflavin-3-gallate theaflavin-3 3-digallate caffeine and theobromine The data show that (1) extraction time from 3 min to 20 min did not significantly affect measured levels of tea compounds and (2) there was a wide variation in the composition of the tea compounds both within each tea category and among categories: black teas contained both theaflavins and catechins green and white teas contained catechins and trace amounts of theaflavins herbal teas contained very low amounts of all tea compounds A statistical profile of the distribution of catechins theaflavins caffeine and theobromine in the evaluated teas offers consumers a choice of teas containing high levels of health-promoting compounds
Article Date November 2005
Issue 9
Volume 70
Key Issues