Technical Abstract Search Details

Technical Abstract Details

Title Trans-cinnamaldehyde and eugenol reduce Salmonella enteritidis colonization in three-week old commercial broiler chickens
Presenter Anup Kollanoor Johny , Tyler Mattson, Sangeetha Ananda Baskaran, Mary Anne Roshni Amalaradjou, Kumar Venkitanarayanan, Michael Darre, Mazhar Khan, Univ of Connecticut, Storrs, CT; Dan Donoghue, Ann Donoghue, Univ of Arkansas, Fayetteville, AR; Jonathan F
Abstract Salmonella enteritidis (SE) is a major food-borne pathogen transmitted through poultry products. Since chickens serve as the reservoir host of SE, reducing the cecal carriage of the bacterium in chickens would decrease contamination of meat and eggs with this pathogen. This study investigated the efficacy of feed supplemented with trans -cinnamaldehyde (TC) or eugenol (EG), two GRAS-status, plant-derived molecules, for reducing SE colonization in 3-week old commercial broiler chickens. In three separate trials, day-old chicks (N=70 per trial) were randomly assigned to five treatment groups (n=14 per group), including positive control (SE alone), 0.5% TC, 0.75% TC, 0.75% EG or 1% EG. Water and feed were provided ad libitum . On day 0, five birds from each trial were sacrificed to confirm that birds were devoid of any inherent Salmonella . On day 8, birds were inoculated with ~ 8.0 log CFU of a 5-strain mixture of nalidixic acid resistant SE by crop gavage. After 24 h, two birds from each group were sacrificed and analyzed to ensure colonization of cecum with the pathogen. Six birds from each group were euthanized on days 7 and 10 after SE challenge, and pathogen populations in the cecum and cloaca were enumerated. Compared to control birds, TC and EG reduced ( P <0.05) SE populations in the cecum by ~ 3 log 10 CFU/g and in the cloaca by ~2 log 10 CFU/g on the 10th day after challenge. Both molecules did not alter the pH or endogenous bacterial counts in the cecum. Feed intake and body weight were not significantly different ( P > 0.05) between TC-treated and control groups. However, EG-treated birds had lower body weights compared to the control ( P < 0.05). The results suggest that supplementation of TC and EG through feed can effectively reduce SE colonization in commercial broiler chickens.
Year/Location2010 IFT Annual Meeting, July 17 - 20, Chicago, IL
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