Technical Abstract Search Details

Technical Abstract Details

Title Plant-derived molecules reduce Salmonella enteritidis adhesion to, and invasion of, intestinal epithelial cells in vitro and down-regulate hilA and hilD expression
Presenter Anup Kollanoor Johny , Kumar Venkitanarayanan, Univ of Connecticut, Storrs, CT
Abstract Salmonella Enteritidis (SE) is a major foodborne pathogen that causes intestinal infections in humans. SE establishes infection in the host by attachment, colonization and invasion of intestinal epithelial cells. Reducing SE attachment and invasion of intestinal cells could decrease the incidence of human illness caused by the pathogen. This study investigated the efficacy of sub-inhibitory concentrations (SICs, concentrations not inhibiting bacterial growth) of four, GRAS-status, plant-derived molecules in reducing the attachment and invasion of cultured intestinal epithelial cells by SE. The molecules and their SICs are trans -cinnamaldehyde (TC, 0.75 mM), eugenol (EG, 3.0 mM), carvacrol (CR, 0.6 mM), and caprylic acid (CA, 10 mM). Confluent monolayers of INT-407 cells (human embryonic intestinal origin) grown in 24-well tissue culture plates (~10 6 cells/well) were inoculated with SE (multiplicity of infection of ~1:80), followed by the addition of plant-molecules at their SICs. Wells were incubated at 37 o C with 5% CO 2 for 2 h, and the number of SE attached to INT-407 cells were determined. In addition, the population of SE that invaded the intestinal cells was enumerated using gentamicin protection assay. In follow up studies, the effect of plant molecules on the expression of invasion gene transcriptional activator, hilA and Salmonella Pathogenicity Island-1 regulator, hilD was investigated, using real-time quantitative PCR. All experiments were replicated three times. All four molecules significantly ( P < 0.05) reduced the adhesion and invasion of intestinal cells by SE. TC, EG, CR, and CA decreased SE invasion of INT-407 cells by 60, 70, 40 and 80%, respectively. Real-time PCR data revealed that all the four molecules significantly ( P < 0.05) down-regulated hilA and hilD expression, compared to controls. Results of this study suggest that TC, EG, CR, and CA could potentially be used to control SE infection, but follow up in vivo studies validating these results are necessary.
Year/Location2010 IFT Annual Meeting, July 17 - 20, Chicago, IL
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