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Technical Abstract Details

Title 238-73 Inhibitory Effect of Achillea millefolium L. Essential Oil on Melanogenesis in Melanoma B16 Cells
Presenter Hsin-Yi Peng, Ying Shih, Yu-Che Lin, Wan-Ching Chan, Yun-Chin Chung, Su-Tze Chou, Providence Univ., Taichung, Taiwan
Abstract Achillea millefolium L. belongs to Asteraoease family. The essential oil (AM-EO) extracted by steam distillation from the flowers parts of Achillea millefolium has reported to exhibit antioxidant and antibacterial activities. However, little information regarding its anti-melanogenesis effect is presently available. In this study, we investigated the effects of AM-EO on melanogenesis, tyrosinase activity, and its gene expression in alphamelanocyte-stimulating hormone (alphaMSH)-stimulated melanoma B16 cells. The transcriptional levels of extracellular signal regulated kinase (ERK) and phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) were also studied. The results indicate that the melanin levels, tyrosinase activity, and tyrosinase expression were decreased after the treatment with AM-EO in alphaMSH-stimulated B16 cells. In addition, the expression levels of p38 MAPK were inhibited by AM-EO. SB203580, a selective inhibitor of p38 MAPK, completely blocked AM-EO-inhibited expression of p38 and tyrosinase in alphaMSH--stimulated B16 cells. The results showed that AM-EO inhibited melanogenesis in B16 cells through the decreased of tyrosinase expression mediated by inhibition of p38 MAPK.
Year/Location2012 IFT Annual Meeting, June 25 - 28, Las Vegas NV
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