Working with a multidisciplinary consortium of 19 researchers from nine institutions, Monell scientists have provided critical information to identify CALHM1 (calcium-homeostasis-modulator-1), a channel in the walls of taste receptor cells, as a necessary component in the process of sweet, bitter, and umami (savory) taste perception.
When sweet, bitter, and umami molecules reach the tongue, they activate taste receptors in specialized cells called Type II taste cells. But how do these taste cells tell the brain they have detected something?
The scientists already knew that activation of taste receptors on Type II cells initiates a complex chain of events inside the taste cells. What they found, as reported in Nature, is that the final step involves the opening of a pore formed by CALHM1 in the taste cell membrane. The open channel allows molecules of the neurotransmitter ATP to leave the taste cell and relay a signal to adjacent nerve cells connected to the brain.
Monell Molecular Neurobiologist Ichiro Matsumato contributed to the work by showing that the gene for CALHM1 is expressed in Type II taste cells, but not in other types of taste tissue. “Our findings demonstrate that the CALHM1 pore is localized specifically in cells that detect sweet, bitter, and umami taste,” said Matsumato.
The necessity of CALHM1 for the ability to taste sweet, bitter, and umami was demonstrated in behavioral tests performed by Monell Taste Biologist Michael G. Tordoff. Reasoning that mice lacking the CALMH1 channel would not be able to release ATP to send information about sweet, bitter, and umami taste detection to the brain, Tordoff tested the taste preferences of Calhm1 ‘knockout’ mice. Engineered by co-author Philippe Marambaud, Feinstein Institute for Medical Research, the knockout mice lack the gene that codes for CALHM1.
“Like humans, mice with an intact CALHM1 gene avidly drink sucrose and other sweeteners, and avoid bitter compounds such as quinine. However, mice lacking CALHM1 are very unusual,” said Tordoff. “These mice treat sweeteners and bitter compounds as if they were water. They behave as if they can’t taste them at all.”
Responses to salty and sour tastes were not affected by the missing gene because perception of these taste qualities is mediated via a different set of taste cells.
The findings demonstrate that pannexins and connexins, channel proteins previously thought to be involved in ATP release from taste cells, actually are not necessary for this to happen. In addition to revealing CALHM1’s critical role in sweet, bitter, and umami taste perception, the current work also lends insight into the channel’s overall function in other tissues. Originally thought to control calcium levels inside cells, CALHM1 may also contribute to ATP-mediated intercellular communication in the brain and elsewhere.